Life Science Research Organisms, plasmids and materials
Protein A affinity chromatography is commonly used in monoclonal antibody (mAb) manufacturing workflows due to its high binding affinity and the purity of the product obtained with it (1). There are occasions when Protein A or its fragments are filtered from a purification column and remain bound to the Fc region of a therapeutic antibody. […]
Description This kit is preferable to the Protein A-h ELISA kit (F050). When the product antibody is human or shows interference with Protein A detection, this kit uses a sample treatment step involving acid dissociation and heat denaturation to overcome any interference from the product immunoglobulin. The LOD is 100 pg/ml. Since this kit was […]
Introduction The Protein A ELISA kit (catalogue number 9000-1) is designed for the detection and quantification of residual native and recombinant protein A (RPA). Our Protein A ELISA kit has been developed for those customers who require a highly sensitive assay to measure small amounts of contaminating protein A in antibody products. Protein A contamination […]
The coronavirus (CoV) spike protein (S), involved in host cell-virus fusion, is the primary immunogenic target for virus neutralization and the current focus of many vaccine design efforts. The highly flexible protein S, with its mobile domains, presents a moving target for the immune system. Here, to better understand protein S mobility, we implement a […]
Interrogating the tumor genome in its entirety by whole-genome sequencing (WGS) presents an unprecedented perception into the biology and pathogenesis of most cancers, with potential influence on diagnostics, prognostication and remedy choice. WGS is ready to detect sequence in addition to structural variants and thereby combines central domains of cytogenetics and molecular genetics. Given the […]
Aegilops tauschii is the diploid progenitor of the D subgenome of hexaploid wheat (Triticum aestivum L.). Right here, the phenotypic knowledge of kernel size (KL), kernel width (KW), kernel quantity (KV), kernel floor space (KSA), kernel width to size ratio (KWL), and hundred-kernel weight (HKW) for 223 A. tauschii accessions had been gathered throughout three steady years. Based […]
To generate a mouse glioblastoma mannequin by genome modifying, we launched Cas9 protein and information RNAs particular for Nf1, Pten, and Trp53 into the neonatal mouse forebrain by electroporation. We discovered a excessive incidence (roughly 90%) of glial tumor improvement, together with glioblastomas, 15 weeks later. The histological options of the tumors had been just like these of diffuse […]
Goals: Lung most cancers in never-smokers is a definite illness related to a distinct genomic panorama, pathogenesis, danger components, and immune checkpoint inhibitor responses in comparison with these noticed in people who smoke. This research aimed to establish novel single nucleotide polymorphisms (SNPs) of programmed death-1 (encoded by PDCD1) and its ligands, programmed demise ligand 1 (CD274) […]
CRISPR interference (CRISPRi) has facilitated the examine of important genes in various organisms utilizing each high-throughput and focused approaches. Regardless of the promise of this system, no complete arrayed CRISPRi library concentrating on important genes exists for the mannequin bacterium Escherichia coli, or for any Gram-negative species. Right here, we constructed and characterised such a […]
In our earlier examine, a intestine microbiota-targeted dietary intervention with a high-fiber weight loss program improved the immune standing of each genetically overweight (Prader-Willi Syndrome, PWS) and easy overweight (SO) kids. Nonetheless, PWS kids had increased irritation ranges than SO kids all through the trial, the intestine microbiota of the 2 cohorts was related. As […]