This kit is preferable to the Protein A-h ELISA kit (F050). When the product antibody is human or shows interference with Protein A detection, this kit uses a sample treatment step involving acid dissociation and heat denaturation to overcome any interference from the product immunoglobulin. The LOD is 100 pg/ml. Since this kit was released, two newer Protein A assays known as Mix-N-Go™ methods have been developed.
These new methods eliminate the sample boiling and centrifugation steps. Instead, a special sample treatment reagent is used that allows accurate detection of Protein A in the presence of its sample antibody. The Protein A Mix-N-Go™ Kit (F600) uses standards calibrated against naturally occurring and conserved recombinant forms of Protein A. The Protein A Mix-N-Go™ Kit (F610) uses standards calibrated against non-naturally occurring Protein A constructs such as MabSelect SuRe™ from GE Healthcare. Use Sample Diluent, Item # I028, with these kits; available separately.
Product Type: ELISA Kit
Storage: All kit reagents 2°C to 8°C
Assay format: 96-well plate
Time to result: ~3 hrs. 20 minutes
LOD: 100 pg/mL
LLOQ: ~200 pg/mL
Recommended thinner: I028