Item Name: E. coli DNA Concentrate, 10ug / mL, 1mL
Biozol catalogue number: CYG-D412-1
Supplier Catalog Number: D412-1
Alternate Catalog Number: CYG-D412-1
Manufacturer: Cygnus Technologies
E. coli DNA concentrate
Storage: 2 ° C to 8 ° C
Each aliquot contains 10 ugs of DNA extracted from a pure culture of Escherichia coli. The identification of this organism was confirmed by 16S sequencing. The purity of the culture was controlled by Gram stain and by additional culture. DNA was extracted from cells following the bacterial protocol in the Qiagen Genomic DNA Manual using Qiagen Genomic DNA Buffers with a 500 / G genomic tip. DNA concentration and A260 / 280 ratios are determined using a NanoDrop ND. -1000. The extracted DNA also tested positive in an internal real-time PCR assay.
The purified genomic DNA is designed for use as an amplification and/or detection control for Escherichia coli nucleic acid testing. It can also be used to determine a limit of detection (LOD), in the development of diagnostic assays, cross-reactivity studies or genomic sequencing. When used as a control for nucleic acid testing, the same protocols as those used to amplify extracted clinical specimens should be used.