The inherent resistance of lignocellulosic biomass makes it impervious for industrially necessary enzymes similar to cellulases to hydrolyze cellulose. Additional, the aggressive absorption conduct of lignin and hemicellulose for cellulases, as a consequence of their electron-rich surfaces augments the inappropriate utilization of those enzymes.

Therefore, modification of the floor cost of the cellulases to cut back its non-specific binding to lignin and improve its affinity for cellulose is an pressing necessity. Additional, sustaining the soundness of cellulases by the preservation of their secondary buildings utilizing immobilization methods may also play an integral function in its industrial manufacturing.

In silico approaches for rising the catalytic exercise of cellulase enzymes can also be important together with a spread of substrate specificity. As well as, enhanced productiveness of cellulases by tailoring the associated genes via the method of genetic engineering and better cellulase restoration after saccharification appears to be promising areas for environment friendly and large-scale enzyme manufacturing ideas.

Our earlier analysis revealed some great benefits of separate feeding (SF) programs in comparison with complete combined ration (TMR) when it comes to ruminal methane (CH₄) manufacturing. The aim of this experiment was to verify the benefit of SF as a dietary technique for CH₄ mitigation, and to find out the consequences of various feeding programs (TMR and SF) on the rumen microbiome and related metagenome of two completely different breeds and on CH₄ emissions.

We randomly allotted 4 Holstein (305 ± 29 kg) and 4 Hanwoo steers (292 ± 24 kg) to 2 teams; the steers have been fed a business focus with tall fescue (75:25) as TMR or SF, in a crossover design (two successive 22-day intervals). Neither feeding programs nor cattle breeds had an impact on the whole tract digestibility of vitamins. The TMR feeding system and Hanwoo steers generated considerably extra CH₄ (P < 0.05) and had the next yield [g/d and g/kg dry matter intake (DMI)] in comparison with the SF system and Holstein steers.

A bigger rumen acetate:propionate ratio was noticed for the TMR than the SF food regimen (P < 0.05), and for Hanwoo than Holstein steers (P < 0.001), clearly reflecting a shift within the ruminal H₂ sink towards CH₄ manufacturing. The linear discriminant evaluation (LDA) impact dimension (LEfSe) revealed a larger abundance (α < 0.05 and LDA > 2.0) of operational taxonomic items (OTUs) associated to methanogenesis for Hanwoo steers in comparison with Holstein steers. Kendall’s correlation evaluation revealed huge variation of microbial co-occurrence patterns between feeding programs, indicating differential H₂ thermodynamics within the rumen.

A metagenome evaluation of rumen microbes revealed the presence of 430 differentially expressed genes, amongst which 17 and 27 genes exhibited optimistic and unfavorable associations with CH₄ manufacturing, respectively (P < 0.001). A robust interplay between feeding system and breed was noticed for microbial and metagenomic abundance.

Total, these outcomes counsel that the TMR feeding system produces extra CH₄, and that Hanwoo cattle are increased CH₄ emitters than SF food regimen and Holstein cattle, respectively. Apparently, host-associated microbial interactions differed inside every breed relying on the feeding system, which indicated that breed-specific feeding programs must be taken under consideration for farm administration.

The event of microbes for conducting bioprocessing through artificial biology includes design-build-test-learn (DBTL) cycles. To help the designing step, we developed a computational approach that means subsequent genetic modifications on the idea of relatedness to the consumer’s design historical past of genetic modifications collected via former DBTL cycles carried out by the consumer.

This system, which comprehensively retrieves well-known designs associated to the historical past, includes looking out textual content for earlier literature after which mining genes that continuously co-occur within the literature with these modified genes. We additional developed a domain-specific lexical mannequin that weights literature that’s extra associated to the area of metabolic engineering to emphasise genes modified for bioprocessing.

Our approach made a suggestion by utilizing a historical past of making a Corynebacterium glutamicum pressure producing shikimic acid that had 18 genetic modifications. Impressed by the suggestion, eight genes have been thought of by biologists for additional modification, and modifying 4 of those genes proved experimentally environment friendly in rising the manufacturing of shikimic acid.

ExFect2000 Transfection Reagent
T202-03	Vazyme	5 ml	EUR 1262.4

PureFection Transfection Reagent
LV750A-1	SBI	1 ml	EUR 303

Transfectamineâ„¢ 5000 Transfection Reagent
60020-05mL	AAT Bioquest	0.5 mL	EUR 222
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ 5000 Transfection Reagent
60021-1mL	AAT Bioquest	1 mL	EUR 334
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ 5000 Transfection Reagent
60022-5mL	AAT Bioquest	5 mL	EUR 846
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ mRNA Transfection Reagent
60030-500ul	AAT Bioquest	500 ul	EUR 203
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells.

Transfectamineâ„¢ mRNA Transfection Reagent
60031-5ml	AAT Bioquest	5 ml	EUR 989
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells.

iMFectin DNA Transfection Reagent
I7100-100	GenDepot	100ul	EUR 176.4

iMFectin DNA Transfection Reagent
I7100-101	GenDepot	1ml	EUR 530.4

iMFectin DNA Transfection Reagent
I7100-105	GenDepot	5x1ml	EUR 2247.6

iMFectin DNA Transfection Reagent
I7100-120	GenDepot	20ml	EUR 8144.4

GeneGlide? DNA Transfection Reagent
M1080-1000	Biovision	each	EUR 636

GeneGlide? DNA Transfection Reagent
M1080-300	Biovision	each	EUR 322.8

GeneGlide? DNA Transfection Reagent
M1080-500	Biovision	each	EUR 433.2

Lentifectin (TM) Transfection Reagent
DNAF003	Bio Basic	1ml	EUR 334.57

Sapphire Insect Transfection Reagent
ABP-BVD-10003	Allele Biotech	75ul, 25 transfections	Ask for price

GeneGlide? siRNA Transfection Reagent
M1081-1000	Biovision	each	EUR 534

GeneGlide? siRNA Transfection Reagent
M1081-300	Biovision	each	EUR 318

GeneGlide? siRNA Transfection Reagent
M1081-500	Biovision	each	EUR 364.8

Transfectamine™ mRNA Transfection Reagent
60030	AAT Bioquest	500 ul	EUR 203

PureFection™ Transfection reagent
LV750A-5	SBI	5 ml	EUR 1287

iMFectin 2000 DNA Transfection Reagent
I7300-101	GenDepot	1ml	Ask for price

iMFectin 2000 DNA Transfection Reagent
I7300-105	GenDepot	5x1ml	Ask for price

iMFectin Poly DNA Transfection Reagent
I7200-100	GenDepot	100ul	EUR 176.4

iMFectin Poly DNA Transfection Reagent
I7200-101	GenDepot	1ml	EUR 530.4

iMFectin Poly DNA Transfection Reagent
I7200-105	GenDepot	5x1ml	EUR 2247.6

iMFectin Poly DNA Transfection Reagent
I7200-120	GenDepot	20ml	EUR 8144.4

T-Pro P-Fect Transfection Reagent
JT97-N005M	T-Pro Biotechnology	1.0ml/vial	EUR 266.4

RNAfection, RNA transfection reagent 50 ul
MR750A-1	SBI	50 ul	EUR 157

T-Pro Nonliposomal Transfection Reagent I (NTR I)
JT97-N001M	T-Pro Biotechnology	1.0ml/vial	EUR 224.4

T-Pro Nonliposomal Transfection Reagent II (NTR II)
JT97-N002M	T-Pro Biotechnology	1.0ml/vial	EUR 244.8

T-Pro Nonliposomal Transfection Reagent III (NTR III)
JT97-N006M	T-Pro Biotechnology	1.0ml/vial	EUR 266.4

293T Transfection Kit (1 mL)
P902	101Bio	-	Ask for price

293T Transfection Kit (0.2 mL)
P902S	101Bio	-	Ask for price

293T Transfection Kit (1 mL)
P903	101Bio	-	Ask for price

293T Transfection Kit (0.2 mL)
P903S	101Bio	-	Ask for price

pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-520A-KIT	SBI	1 kit	EUR 1141

pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-550A-KIT	SBI	1 kit	EUR 1141

pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-575A-KIT	SBI	1 kit	EUR 1141

pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-599A-KIT	SBI	1 kit	EUR 1141

pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-639A-KIT	SBI	1 kit	EUR 1141

pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-649A-KIT	SBI	1 kit	EUR 1141

pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-659A-KIT	SBI	1 kit	EUR 1141

pPACK-SPIKE Omicron Combo Kit, includes Cat# CVD19-660A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-669A-KIT	SBI	1 kit	EUR 1162

pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-619A-KIT	SBI	1 kit	EUR 1141

pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-629A-KIT	SBI	1 kit	EUR 1141

pPACK-SPIKE B.1.351 RBD Mutations Combo Kit, includes Cat# CVD19-580A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-589A-KIT	SBI	1 kit	EUR 1141

pPACK-SPIKE B.1.351 S1 Mutations Combo Kit, includes Cat# CVD19-600A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
CVD19-609A-KIT	SBI	1 kit	EUR 1141

FBS, Transfection Optimized
F0640-050	GenDepot	500ml	EUR 902.4

Calcium Phosphate Transfection Kit
GR103070	Genorise Scientific	100 preps	EUR 219

Protoplast Solution for Transfection
GR103052	Genorise Scientific	10 mL	EUR 119

Exo-Fect Exosome Transfection Kit
EXFT10A-1	SBI	10 reactions	EUR 238

Exo-Fect Exosome Transfection Kit
EXFT20A-1	SBI	20 reactions	EUR 429

Exo-Fect siRNA/miRNA Transfection Kit
EXFT200A-1	SBI	20 reactions	EUR 412

Transfection-ready Cas9 SmartNuclease AAVS1 gRNA
CAS520A-1	SBI	10 ug	EUR 281

Transfection Collection™ : PDE4D Transient Pack
79286	BPS Bioscience	500 rxns.	EUR 845
Description: The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells._x000D_The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter._x000D_However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. The PDE4D Transient Pack contains transfection-ready CRE luciferase reporter. This reporter contains transfection-ready vectors for PDE4D7 expression and a CRE vector containing firefly luciferase as a cAMP pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control._x000D_It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the PDE4D Cell-Based Activity Reporter Detection Valuepack is the CRE luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. The vector is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The pack also includes the PDE4D7 expression vector, and the adenylate cyclase activator, forskolin. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep._x000D_Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth

Phosphate-buffered Sucrose for Transfection
GR103050	Genorise Scientific	1 L	EUR 119

Plant Electroporation Buffer for Transfection
GR103051	Genorise Scientific	1 L	EUR 119

Sapphire Baculovirus DNA and Transfection Kit
ABP-BVD-10002	Allele Biotech	1 kit	Ask for price

NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA)
DR5001-HNHela-Sce2	TopoGen	100ug DNA	EUR 3486

NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA)
DR5001-HNHela-Sce3	TopoGen	250ug DNA	EUR 4110

HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA)
DR3000-HRHeLa-CPR2	TopoGen	100ug DNA	EUR 3610.8

HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA)
DR3000-HRHeLa-CPR3	TopoGen	250ug DNA	EUR 4234.8

HR based Kit in HeLa Requiring I-Sce1 Transfection (100ug DNA)
DR3000-HRHeLa-Sce2	TopoGen	100ug DNA	EUR 3486

HR based Kit in HeLa Requiring I-Sce1 Transfection (250ug DNA)
DR3000-HRHeLa-Sce3	TopoGen	250ug DNA	EUR 4110

100 x DEAE-dextran Stock Solution for Transfection
GR103049	Genorise Scientific	10 x 10 mL	EUR 119

Transfection-ready Cas9 SmartNuclease mRNA (Eukaryotic version)
CAS500A-1	SBI	20 ug	EUR 387

NHEJ Based HeLa Requires I-Sce1 Transfection (50ug DNA)
DR5001-HNHela-Sce	TopoGen	50ug DNA	EUR 3174

"N" SARS-CoV-2 Nucleocapsid mRNA (10 µg, Transfection-ready)
CVD19-420A-1	SBI	10 µg	EUR 328

"N" SARS-CoV-2 Nucleocapsid mRNA (20 µg, Transfection-ready)
CVD19-425A-1	SBI	20 µg	EUR 468

HR in human Neuroblastoma Cells req. I-Sce1 Transfection (100ug DNA)
DR3000-HRSH-Sce2	TopoGen	100ug DNA	EUR 3735.6

HR in human Neuroblastoma Cells req. I-Sce1 Transfection (250ug DNA)
DR3000-HRSH-Sce3	TopoGen	250ug DNA	EUR 4359.6

NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (100ug DNA)
DR5000-HN3T3-Sce2	TopoGen	100ug DNA	EUR 3486

NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (250ug DNA)
DR5000-HN3T3-Sce3	TopoGen	250ug DNA	EUR 4110

"S" SARS-CoV-2 Spike Omicron mRNA (10 µg, Transfection-ready)
CVD19-440A-1	SBI	10 µg	EUR 383

"S" SARS-CoV-2 Spike Omicron mRNA (20 µg, Transfection-ready)
CVD19-445A-1	SBI	20 µg	EUR 553

HR based Kit in HeLa Requiring CRISPR Transfection (50ug DNA)
DR3000-HRHeLa-CPR	TopoGen	50ug DNA	EUR 3298.8

HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (100ug DNA)
DR3000-HR3T3-Sce2	TopoGen	100ug DNA	EUR 3486

HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (250ug DNA)
DR3000-HR3T3-Sce3	TopoGen	250ug DNA	EUR 4110

HR based Kit in HeLa Requiring I-Sce1 Transfection (50ug DNA)
DR3000-HRHeLa-Sce	TopoGen	50ug DNA	EUR 3174

"S" SARS-CoV-2 Spike Glycoprotein mRNA (10 µg, Transfection-ready)
CVD19-430A-1	SBI	10 µg	EUR 360

"S" SARS-CoV-2 Spike Glycoprotein mRNA (20 µg, Transfection-ready)
CVD19-435A-1	SBI	20 µg	EUR 520

Transfection Collection™ : AP1 Transient Pack (JNK Signaling Pathway)
79266	BPS Bioscience	500 rxns.	EUR 715
Description: The AP1 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a JNK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the AP1 Transient Pack is the AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter. The AP1 reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth

Transfection Collection™ : Myc Transient Pack (Myc Signaling Pathway)
79284	BPS Bioscience	500 rxns.	EUR 750
Description: The Myc Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Myc signaling pathway in cultured HCT116 cells. The Myc Transient Pack contains transfection-ready vectors containing firefly luciferase as a Myc pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HCT116 cells._x000D_.The key to the Myc Transient Pack is the expression vectors for c-Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. This negative control is critical for determining pathway-specific effects and background luciferase activity. An "empty" expression vector without the c-Myc gene is also provided as an additional negative control._x000D_Additionally, the pack includes cell culture medium that has been optimized for use with HCT116 cells*. HCT116 is a human colon cancer cell line with a mutated β -catenin which leads to the accumulation of β-catenin and constitutive activation of the Myc signaling pathway. This medium includes 10% fetal bovine serum, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HCT116, but an alternate cell culture medium may be required for optimal cell growth. For HEK293 cells, we recommend using BPS Medium 1 (BPS Bioscience, #79259).

HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (100ug DNA)
DR3000-HRCN1.4-Sce2	TopoGen	100ug DNA	EUR 3735.6

HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (250ug DNA)
DR3000-HRCN1.4-Sce3	TopoGen	250ug DNA	EUR 4359.6

NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (100ug DNA)
DR5000-HNSH-Sce2	TopoGen	100ug DNA	EUR 3610.8

NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (250ug DNA)
DR5000-HNSH-Sce3	TopoGen	250ug DNA	EUR 4234.8

NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (100ug DNA)
DR5000-HNCN1.4-Sce2	TopoGen	100ug DNA	EUR 3486

NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (250ug DNA)
DR5000-HNCN1.4-Sce3	TopoGen	250ug DNA	EUR 4110

HR in human Neuroblastoma Cells req. I-Sce1 Transfection (50ug DNA)
DR3000-HRSH-Sce	TopoGen	50ug DNA	EUR 3423.6

NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (50ug DNA)
DR5000-HN3T3-Sce	TopoGen	50ug DNA	EUR 3174

Transfection Collection™ : SRE Transient Pack (MAPK/ERK Signaling Pathway)
79271	BPS Bioscience	500 rxns.	EUR 750
Description: The SRE Reporter Kit Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRE in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a MAPK/ERK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SRE Transient Pack is the SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth.

Transfection-ready Cas9 SmartNickase mRNA (Eukaryotic Nickase mutant version)
CAS504A-1	SBI	20 ug	EUR 387

Transfection Collection™ : SBE Transient Pack (TGFβ/SMAD Signaling Pathway)
79272	BPS Bioscience	500 rxns.	EUR 750
Description: The SBE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a TGFβ/SMAD pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SBE Transient Pack is the SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection Collection™ : NF-κB Transient Pack ( NF-κB Signaling Pathway)
79268	BPS Bioscience	500 rxns.	EUR 780
Description: The NF-κB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the NF-κB signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a NF-κB pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the NF-κB Transient Pack is the NF-κB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-κB responsive element located upstream of a minimal promoter. The NF-κB reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

HR based Kit in MOUSE 3T3 Cells Requiring I-Sce1 Transfection (50ug DNA)
DR3000-HR3T3-Sce	TopoGen	50ug DNA	EUR 3174

Transfection Collection™ : Notch1/CSL Transient Pack (Notch Signaling Pathway)
79285	BPS Bioscience	500 rxns.	EUR 715
Description: The Notch1/CSL Transient Packis designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Notch signaling pathway in HEK293 cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a Notch pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the Notch1/CSL Transient Pack is the expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1DE). Inside the cells, the NOTCH1 DE can be cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth,

The Transfection Collection™ : ISRE Transient Pack JAK/STAT Signaling Pathway
79264	BPS Bioscience	500 rxns.	EUR 715
Description: The ISRE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in cultured HEK293 cells. The pack contains transfection-ready vectors containing firefly luciferase as a JAK/STAT pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the ISRE Transient Pack is the ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)
P402	101Bio	-	Ask for price

HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (50ug DNA)
DR3000-HRCN1.4-Sce	TopoGen	50ug DNA	EUR 3423.6

Transfection Collection™ : CRE/CREB Transient Pack (cAMP/PKA Cell Signaling Pathway)
79267	BPS Bioscience	500 rxns.	EUR 715
Description: The CRE/CREB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the cAMP/PKA signaling pathway in cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a cAMP/PKA pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the CRE/CREB Transient Pack is the CRE/CREB luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase._x000D_The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with the constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth.

NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (50ug DNA)
DR5000-HNSH-Sce	TopoGen	50ug DNA	EUR 3298.8

NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (50ug DNA)
DR5000-HNCN1.4-Sce	TopoGen	50ug DNA	EUR 3174

The Transfection Collection™ : GAL4 Transient Pack Glucocorticoid Receptor Pathway
79265	BPS Bioscience	500 rxns.	EUR 715
Description: The GAL4 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the glucocorticoid signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a glucocorticoid pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the GAL4 Transient Pack is the expression vector for the glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection Collection™ : TCF/LEF Transient Pack Wnt / β-catenin Signaling Pathway
79273	BPS Bioscience	500 rxns.	EUR 750
Description: The TCF/LEF Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of Wnt / Beta-catenin signaling pathway in the cultured HEK293 cells. The TCF/LEF Transient Pack contains transfection-ready vectors containing firefly luciferase as a Wnt pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the TCF/LEF Transient Pack is the TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth.

Transfection-ready hspCas9-T2A-GFP SmartNuclease mRNA (wildtype Cas9 with GFP marker)
CAS530G-1	SBI	10 ug	EUR 342

Transfection-ready hspCas9-T2A-RFP SmartNuclease mRNA (wildtype Cas9 with RFP marker)
CAS531R-1	SBI	10 ug	EUR 342

Transfection-ready hspCas9-nickase-T2A-GFP SmartNickase mRNA (Cas9 Nickase mutant with GFP marker)
CAS534G-1	SBI	10 ug	EUR 342

Transfection-ready hspCas9-nickase-T2A-RFP SmartNickase mRNA (Cas9 Nickase mutant with RFP marker)
CAS535R-1	SBI	10 ug	EUR 342

EP Reagent Lead Sol. 0.1% Pb
5001700	Scientific Laboratory Supplies	100ML	EUR 148.8

Bottle Reagent 1000 ml Pyrex
BOT5356	Scientific Laboratory Supplies	PK10	EUR 177.6

Sample Transport Reagent
SSFIN-0007	Zeptometrix	20mL	EUR 32

EP Reagent Copper Std Sol. 0.1% Cu
5001100	Scientific Laboratory Supplies	100ML	EUR 142.8

Reagent reservoir 50 mL
DD53482	Scientific Laboratory Supplies	PK100	EUR 230.4

50 ml Reagent Reservoir
GR238016	Genorise Scientific	Pack of 200	EUR 148

TransDux virus transduction reagent (200x)
LV850A-1	SBI	80 transductions	EUR 257

REAGENT BOTTLE, 250 ML, PP
1500P-250	CORNING	20/pk	EUR 103.2
Description: Reusable Plastics; Resusable Bottles

REAGENT BOTTLE,500 ML, PP
1500P-500	CORNING	10/pk	EUR 74.4
Description: Reusable Plastics; Resusable Bottles

Reagent reservoir 50 mL Treff
DD34102	Scientific Laboratory Supplies	PK10	EUR 73.2

Genorise® GSI RNA Reagent, 200 ml
GR102015	Genorise Scientific	200	EUR 342

Genorise® GSI RNA Reagent, 100 ml
GR102034	Genorise Scientific	100	EUR 193

EP Reagent Biuret Reagent
1011601	Scientific Laboratory Supplies	1L	EUR 52.8

EP Reagent Iodoplatinate Reagent
1046300	Scientific Laboratory Supplies	200ML	EUR 446.4

AAVancedTM Concentration Reagent (100 ml aliquot)
AAV100A-1	SBI	100 ml	EUR 462

AAVancedTM Concentration Reagent (250 ml aliquot)
AAV110A-1	SBI	250 ml	EUR 928

EP Reagent Molybdovanadic Reagent
1056700	Scientific Laboratory Supplies	100ML	EUR 52.8

Traut's Reagent
2330-1000	Biovision	each	EUR 418.8

Traut's Reagent
2330-500	Biovision	each	EUR 248.4

Protein Detection Reagent unit: 100 ml
FYP202-100ML	Yeastern Biotech	100ml	Ask for price

EP Reagent Methoxyphenylacetic Reagent
1053601	Scientific Laboratory Supplies	100ML	EUR 435.6

EP Reagent Conc to make Cadmium Std Sol. 0.1% Cd
5000700	Scientific Laboratory Supplies	100ML	EUR 265.2

Genorise® GSI RNA Reagent, 50 ml
GR102035	Genorise Scientific	50	EUR 117

These outcomes indicated that our proposed approach efficiently utilized the previous cycles to counsel related designs that biologists thought of price testing. Complete retrieval of well-tested designs will assist less-experienced researchers overcome the entry barrier in addition to encourage skilled researchers to formulate design ideas which have been neglected or suspended. This system will support DBTL cycles by feeding histories again to the subsequent genetic design, thereby complementing the designing step.